About E.M. (Emma) de Pater, PhD
Field(s) of expertise
Recently, a Gata2 haploinsufficiency model in zebrafish was generated, mimicking patient characteristics like monocytopenia and the development of dysplasia. Furthermore, transgenic lines for screening purposes are generated. The lab uses various techniques to undestand the mechanism of malignant transformation like genome editing, immunohistochemistry, FACS, live confocal imaging and bulk- and single cell transcriptomics.
Education and career
Emma studied Biology in Leiden and obtained her PhD under the supervision of Prof. Dr. Bakkers at the Hubrecht Institute (Utrecht). She gained extensive experience in the use of the zebrafish system both at the Hubrecht Institute and at the Max Planck Institute in Tübingen in the laboratory of Nobel Laureate Prof. Dr. Nüsslein-Volhardt where she participated in a forward genetic screen for cardiac mutants. During her PhD she showed the contribution of the second heartfield to cardiac growth in zebrafish and uncovered the importance of the transcription factor Islet-1 for sinoatrial node formation.
In the laboratory of Prof. Dr. Dzierzak (Erasmus MC), she studied the role of the transcription factor Gata2 in embryonic hematopoiesis. She now combines this experience to study the role of germline mutations, specifically GATA2, in the development of MDS and AML. Both mouse and zebrafish models are used to study the onset and mechanism of hematological malignancy resulting from bone marrow failure syndromes.
In the laboratory of Prof. Dr. Dzierzak (Erasmus MC), she studied the role of the transcription factor Gata2 in embryonic hematopoiesis. She now combines this experience to study the role of germline mutations, specifically GATA2, in the development of MDS and AML. Both mouse and zebrafish models are used to study the onset and mechanism of hematological malignancy resulting from bone marrow failure syndromes.
Publications
Top 5 publications:
De Pater E* & Trompouki E*, Bloody zebrafish – Emerging methods in the study of normal and malignant hematopoiesis. Frontiers in Cell and Developmental Biology. (DOI: 10.3389/fcell.2018.00124).
Kaimakis P*, De Pater E*, Eich C*, Solaimani Kartalaei P, Kauts ML, Vink CS, van der Linden R, Jaegle M, Yokomizo T, Meijer D, Dzierzak E. Functional and molecular characterization of mouse Gata2-independent hematopoietic progenitors. Blood. 2016 Feb 1. pii: blood-2015-10-673749
de Pater E, Kaimakis P, Vink C, Yokomizo T, Yamada-Inagawa T, Van der Linden R, Camper S, Speck N and Dzierzak E. Gata2 is required for HSC generation and survival. Journal of Experimental Medicine, 2013 Dec;210(13):2843-50.
de Pater E, Ciampricotti M, Priller F, Veerkamp J, Strate I, Smith K, Lagendijk AK, Schilling TF, Herzog W, Abdelilah-Seyfried S, Hammerschmidt M, Bakkers J. Bmp signaling exerts opposite effects on cardiac differentiation, Circ Res. 2012 Feb 17;110(4):578-87
de Pater E, Clijsters L, Marques SR, Lin YF, Garavito-Aguilar ZV, Yelon D, Bakkers J. Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart. Development. 2009 May;136(10):1633-41.
For more information, see Pubmed
De Pater E* & Trompouki E*, Bloody zebrafish – Emerging methods in the study of normal and malignant hematopoiesis. Frontiers in Cell and Developmental Biology. (DOI: 10.3389/fcell.2018.00124).
Kaimakis P*, De Pater E*, Eich C*, Solaimani Kartalaei P, Kauts ML, Vink CS, van der Linden R, Jaegle M, Yokomizo T, Meijer D, Dzierzak E. Functional and molecular characterization of mouse Gata2-independent hematopoietic progenitors. Blood. 2016 Feb 1. pii: blood-2015-10-673749
de Pater E, Kaimakis P, Vink C, Yokomizo T, Yamada-Inagawa T, Van der Linden R, Camper S, Speck N and Dzierzak E. Gata2 is required for HSC generation and survival. Journal of Experimental Medicine, 2013 Dec;210(13):2843-50.
de Pater E, Ciampricotti M, Priller F, Veerkamp J, Strate I, Smith K, Lagendijk AK, Schilling TF, Herzog W, Abdelilah-Seyfried S, Hammerschmidt M, Bakkers J. Bmp signaling exerts opposite effects on cardiac differentiation, Circ Res. 2012 Feb 17;110(4):578-87
de Pater E, Clijsters L, Marques SR, Lin YF, Garavito-Aguilar ZV, Yelon D, Bakkers J. Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart. Development. 2009 May;136(10):1633-41.
For more information, see Pubmed
Other positions
Manager Cancer Genome Editing Center, Erasmus MC.
Scholarships, grants, and awards
- Dec 2016 : KWF/Alpe d’Huzes junior fellowship, Bas Mulder award
- Dec 2014 : EHA non clinical junior research fellowship
More
Particpating in the "the Pater Research Group".
